Figure 4.

The level of heavily glycosylated HAI-2 is disproportionally low in LoVo colorectal adenocarcinoma cells that harbor SPINT2 mutation. Lysates containing equal amounts of protein from four colorectal cancer lines, as indicated, were analyzed by immunoblot for the expression of lightly glycosylated HAI-2 using the mAb XY9 (A) and heavily glycosylated HAI-2 using the mAb DC16 (B). The various HAI-2 species are indicated. The ratios of lightly versus heavily glycosylated HAI-2 for each cell line were estimated from the band density using three independent experiments using ImageJ (C). ^***P < 0.001.