Figure 7.

The SCSD-associated SPINT2 mutants are not expressed as heavily glycosylated HAI-2. HAI-2 protein expression was induced using different concentrations of doxycycline (Dox.), as indicated, in HAI-2 knockout Caco-2 variants engineered to express wild-type HAI-2 (A) and the three SCSD-associated mutants, R148C HAI-2 (B), R148H HAI-2 (C), and Y163C HAI-2 (D). Equal amounts of cellular proteins were analyzed by immunoblot under non-reducing and non-boiled conditions for HAI-2 species using the HAI-2 mAb DC16. The various HAI-2 species were indicated.