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. 2024 Jan 27;33(9):787–801. doi: 10.1093/hmg/ddae011

Figure 2.

Figure 2

EZH2 targets the Lbx1 locus for repression. (A) RegulomeDB (2.0) analysis for chr10:102979206-102 979 207 found that this region is in a repressed chromatin state for most cell types and tissues. (B) ChIP-qPCR for EZH2 (n = 2). (C) The expression of Lbx1 mRNA was higher in the neural tube at E12.5, compared to that in the developing brain (n = 3-4), two-tailed unpaired t-test. One-way ANOVA with Tukey’s multiple comparison test. ***P < 0.001, **P < 0.01. Alt. Text. A. Three horizontal bar graphs showing the chromatin state for 127 tissues and cell 29 types. For 123 of these, the region of interest is in a region repressed by polycomb. Panel B: 30 vertical bar graph showing the percentage pull-down of EZH2 bound to the AIS-CRM genomic 31 region or the transcriptional start site. The data shows ~5-fold increase in bound EZH2 for 32 chromatin extracted from brain tissue compared to the neural tube. Panel C: Vertical bar 33 graph showing that Lbx1 mRNA expression is near 0 for the brain and ~0.25 (relative gene 34 expression) in the neural tube.