Figure 4.

SpiderMass classification based on immune cell lipid profiles

(A) Trained LGBM model for cancer cells, lymphocytes, and M1-like and M2-like macrophages that were analyzed previously by SpiderMass technology after isolation.

(B) Boxplot of three biomarkers for GBM cancer cells, lymphocytes, and M1-like and M2-like macrophages.

(C) The overall pipeline for seeing the distribution of cancer and immune cells in GBM tissue analyzed by SpiderMass-MSI.

(D) Multiplex MALDI-IHC on three biomarkers in two GBM fresh frozen tissue sections. The display scale (arbitrary peak intensity units) is as follows (minimum intensity/full intensity threshold): 4/25 (Ki67), 2/6 (CD8α), and 4/25 (CD68).

ns, not significant: p ≤ 0.05; ∗p ≤ 0.01; ∗∗p ≤ 0.001; ∗∗∗p ≤ 0.0001.

Related to Table S8.