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. 2024 Apr 11;30(4):990–1000. doi: 10.1038/s41591-024-02848-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — (a) Immunofluorescence analysis confirming the presence of pathology markers myogenin and desmin in EV004-RMS. Images taken at 90x using a laser scanning confocal microscope (Fluoview FV10i, Olympus) utilizing the FV10 image software. Representative images of one independent experiment due to limited PDC material. (b) Comparison of genomic alterations detected in UCSF500 tumor panel profiling with genomic profiling of original tumor sample at enrollment (T) and PDC at time of DST for EV002-AML, EV004-RMS, EV007-GBM, EV009-OS, EV013-AML, EV019-MB, EV023-ALL. Color code on the left indicates type of variant identified from UCSF500 profiling.