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. 2024 Apr 11;30(4):990–1000. doi: 10.1038/s41591-024-02848-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 2 — Immune cell type deconvolution and tumor purity analysis was done from original tissue (T) and PDCs (when available). a) Analysis of EV004-RMS. Bulk RNA-seq was deconvoluted using the analysis tools EPIC (Top Left, T and PDC) and quanTIseq (Bottom Left, T and PDC). Immune cell composition (T and PDC) was analyzed using TIMER (Top Right). Tumor purity analysis was done using pathology analysis (T, in green), ESTIMATE (T and PDC, in blue), quanTIseq (T and PDC, in purple), and EPIC (T and PDC, in yellow). A similar approach was used in b) EV009-OS, c) EV007-GBM, d) EV002-AML and e) EV013-AML.