TABLE 1.
Summary sequencing data for WT and dwfcp
| Sequencing results | WT | dwfcp | ||||
| No. of reads | 95 993 772 | 84 920 814 | ||||
| Mapped reads (%) | 98.09 | 98.28 | ||||
| Average depth | 44.49 | 39.45 | ||||
| Coverage at least 4× (%) | 95.37 | 94.86 | ||||
| SNP filtering | ||||||
| Total no. of SNPs | 425 994 | 417 882 | ||||
| AF (WT) ≤ 0.6; AF (dwfcp) = 1 | 1126 | 1126 | ||||
| EMS SNPs G > A or C > T | 317 | 317 | ||||
| EMS SNPs (GQ > 75; DP > 20) | 2 | 2 | ||||
| High-impact SNPs | 0 | 1 | ||||
| Candidate SNPs | ||||||
| Chr | Position | Ref. | Alt | Gene ID | Effect | Functional annotation |
| 17 | 3.048.864 | G | A | Cp4.1LG17g04540 | Splice site mutation | 7-Dehydrocholesterol reductase |
DP, read depth; GQ, genotype quality.