TABLE 2.
Comparison of the genetic variability between the pol sequences of HIV-1 groups O and M
| HIV-1 group, isolate, source, and no. | Subtype classification | Regiona | Mutation frequencyb | % Nucleotide diversityc
|
|
|---|---|---|---|---|---|
| Average ± SD | Range | ||||
| O | |||||
| 4 Spanish isolates (this study) | Total | 1–560 | 1.1 × 10−2 | 2.2 ± 0.5 | 1.6–2.8 |
| 4.4 × 10−2 | 7.8 ± 2.8 | 1.4–12.9 | |||
| 16 isolates (13) (this study) | Subtype A-O | 1–264 | 2.9 × 10−2 | 5.0 ± 1.4 | 1.4–7.1 |
| Non-subtype A-O | 7.8 × 10−2 | 9.0 ± 1.5 | 5.7–12.9 | ||
| Quasispecies (this study) | 28–116 | 6.5 × 10−3 | 1.1 ± 0.6 | 0–2.4 | |
| M | |||||
| Subtype B (49) | Total | 1–560 | 1.9 × 10−2 | 3.5 ± 0.7 | 0.8–5.4 |
| 5.0 × 10−2 | 8.9 ± 3.3 | 1.8–14.4 | |||
| 10 isolates (40) | Subtype B | 1–264 | 2.7 × 10−2 | 4.9 ± 1.1 | 1.8–4.1 |
| Non-subtype B | 6.5 × 10−2 | 10.6 ± 2.7 | 2.8–14.4 | ||
| Quasispecies (41, 49) | 41–108/181–219 | 7.9 × 10−3 | 1.4 ± 1.0 | 0–5.4 | |
a
Numbers correspond to codons in the RT. The full RT is 560 amino acids in length, while the polymerase domain spans codons 1 to 266.
b
Mutation frequency is defined as the proportion of mutant positions relative to the consensus nucleotide sequence for each sample group; the frequencies have been calculated by dividing the number of mutations (relative to the consensus) by the total number of nucleotides sequenced in the corresponding group.
c
Corresponds to the average proportion of substituted nucleotides when pairs of sequences were compared by applying the Kimura two-parameter model (26).