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. 2024 Apr 19;22:233. doi: 10.1186/s12964-024-01612-y

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Mutations of F278A and F281A ameliorates 5-HT7R-induced Tau pathology. A, B Phosphorylation levels of endogenous CDK5 in N1E-115 expressing HA-tagged 5-HT7R WT or indicated phenylalanine mutants. Representative Western blot (A) and quantification (B). Data is represented as mean ± SD (N = 4, one-way ANOVA, Dunnett’s multiple comparisons, * p < 0.05). C, D. N1E-115 cells were transfected with eGFP-Tau[R406W] mutant, together with indicated HA-tagged 5-HT7R constructs. Phospho-Tau and total Tau levels were detected with AT270 and 5A6 antibodies, respectively. Resulting ratios (D) were normalized to GAPDH expression and are shown as normalized mean ± SD (N = 3, one-way ANOVA, Dunnett’s multiple comparisons, * p < 0.05). E The number of Tau aggregate-positive cells was counted in a confined area and is presented as a fraction of the total number of transfected cells. Data is presented as normalized mean ± SD (N = 3, n ≥ 353, Dunnett’s multiple comparisons, ** p < 0.01, *** p < 0.001)