Fig. 1.

A photo of PhenoWorld and experimental procedures. 7–8 weeks old of Wistar rats were held in quarantine for 7 days, afterwards moved to conventional housing room. After 7 days of adaptation period here, unstressed animals were only handled, stressed groups were exposed to chronic unpredictable stress for 4 weeks. Home-cage behaviors were recorded for total of 10 days during the experimental period. After this period, blood samples were obtained for corticosterone analyses. EPM test was conducted, the animals were killed at the end of the experiment and tissue samples were obtained.