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The entire HIV-1 gp120 sequence (511 amino acids) showing the regions of interest, including the V1-V2 and C2-V3 fragments and restriction sites used in this study. The V1 and V2 regions lie between the DraIII and StuI sites (76 amino acids), and the C2 and V3 regions are within the StuI-to-NheI fragment (143 amino acids).
