FIG. 5.

Expression of HIV-1 coreceptors CXCR4 and CCR5 on costimulated adult PBL. CD4-depleted adult PBL were analyzed for CCR5 (FITC), CXCR4 (PE), CD8 (allophycocyanin), and CD4 (red 613) 3 days following costimulation with anti-CD3 and anti-CD28 MAbs. Coreceptor expression on the different CD8-expressing subsets was quantitated by gating. Gates are denoted by black boxes in the left column. Cells within the different gates expressing CCR5 and CXCR4 are represented on the right. The percentage of cells single positive (SP) or double positive (DP) for the indicated marker is given within each quadrant.