Fig. 5.

ABC transporter mutants dominate as fitness factors both in vivo and ex vivo and result in unique in vitro phenotypes. (A) Twelve ABC operons were found to have multiple mutants displaying either infection-specific (blue), host-specific (green), organ-specific (purple), or growth (yellow) defects. SI Appendix, Fig. S6 has a flow diagram for Tn mutant binning and Dataset S4 has all the binning assignments for each mutant. These entire transport systems were deleted using Lambda Red Recombinase. (B) These mutants were tested for growth in LB, human urine (HU), and MOPS supplemented with glucose (0.2%), glycerol (0.2%), or casamino acids (0.4%) as a sole carbon source. An iron-free MOPS medium containing glucose was also tested with 0.2% glucose. Area under the curve was determined for each mutant in all media formulations and is displayed on the heat map. One-way ANOVA was performed using Dunnett’s multiple-test correction compared to WT CFT073. *P < 0.05, **P < 0.01, ***P < 0.001. (C) Mutants were also tested for motility in semisoft (0.25%) agar. Following incubation at 30 °C, swim diameters (mm) were measured. One-way ANOVA was performed using Dunnett’s multiple-test correction compared to WT CFT073. **P < 0.01, ****P < 0.0001.