Fig. 2.
Detection of enzyme activity in T. kodakarensis cell extracts. (A) 2-PG phosphatase activity in cell extracts from T. kodakarensis KU216, ΔTK1734, and ΔTK2301 strains. Gray or white bars indicate the specific activities in extracts from cells grown under anaerobic or microaerobic conditions, respectively. (B) GLDH activity from glycolate to glyoxylate in cell extracts from KU216, ΔTK0551, and ΔTK0683 strains. Gray or white bars indicate the specific activities in extracts from cells grown under anaerobic or microaerobic conditions, respectively. (C) GLDH activity from glyoxylate to glycolate in cell extracts from KU216, ΔTK0551, and ΔTK0683 strains. Gray or white bars indicate the specific activities in extracts from cells grown under anaerobic or microaerobic conditions, respectively. (D) AGAT activity in cell extracts from KU216, ΔTK0186, ΔTK0548, ΔTK2268, and ΔTK1094 strains. Gray or white bars indicate the specific activities in extracts from cells grown under anaerobic or microaerobic conditions, respectively. Activity measurements were carried out as described in the Methods section at 80 °C. The data represent the average of three independent experiments and are shown with the SD values. 0.01 < P ≤ 0.05*; 10−3 < P ≤ 10−2**; 10−4 < P ≤ 10−3***; 10−5 < P ≤ 10−4****; 10−7 < P ≤ 10−6******; 10−8 < P ≤ 10−7*******.