Fig. 3.

Phosphatase, dehydrogenase, or aminotransferase activity of recombinant proteins with various substrates. Phosphatase activities of the purified TK1734 (A) and TK2301 (B) proteins were measured toward various phosphorylated metabolites. Dehydrogenase activities of the purified TK0551 (C) and TK0683 (D) proteins were examined toward various 2-hydroxyacids. Aminotransferase activities of the purified TK0186/TK1094 proteins with various amino donor compounds in the presence of glyoxylate as the amino acceptor (E/G) or various amino acceptor compounds in the presence of alanine as the amino donor (F/H) were evaluated. In all cases (A–H), activity measurements were carried out as described in the Methods section at 80 °C. ND: not detected. The data represent the average of three independent experiments and are shown with the SD values.