Table 1.
Enzyme | Substrate |
K m (mM) |
k cat (s−1) |
kcat/Km (s−1 mM−1) |
---|---|---|---|---|
TK1734 | 2-PG | 1.75 ± 0.22 | 65.6 ± 2.5 | 37.5 |
TK2301 | 2-PG | 1.22 ± 0.09 | 21.2 ± 0.4 | 17.4 |
E4P | 1.25 ± 0.28 | 17.1 ± 1.0 | 13.7 | |
Ru5P | 0.979 ± 0.157 | 16.3 ± 0.5 | 16.6 | |
RuBP | 0.238 ± 0.044 | 30.1 ± 1.3 | 126 |
All analyses were carried out at 80 °C.