Table 1.

Details of the MD simulations^*

	CHL1	POPC	SAGL	SAPE	SAPI2D	SDPE	SDPS	SOPS	Total
Vesicle
Outer leaflet	1,266	296	0	533	0	283	211	199	2,791
%	45.4	10.6	0	19.1	0	10.1	7.6	7.1
Inner leaflet	826	669	0	184	0	99	1	1	1,780
%	46.4	37.6	0	10.3	0	5.6	0.1	0.1
Flat bilayer 1
Upper leaflet	748	130	17	115	82	231	165	161	1,649
%	45.3	7.9	1.0	7.0	5.0	14.0	10.0	9.8	100
Lower leaflet	720	688	16	64	0	112	0	0	1,600
%	45.0	43.0	1.0	4.0	0	7.0	0	0	100
Flat bilayer 2
Upper leaflet	540	96	12	84	60	156	120	120	1,188
%	45.4	8.1	1.0	7.1	5.1	13.1	10.1	10.1
Lower leaflet	540	516	12	36	0	84	0	0	1,188
%	45.4	43.4	1.0	3.0	0	7.1	0	0
System name	Flat bilayer			No. of atoms			Initial cell dimensions		Length and temperature
				4,390,099			35 × 35 × 35 nm3		887 ns 310 K / 805 ns 350 K
t350f2e				5,266,689			37.5 × 37.5 × 37.5 nm3		780 ns 350 K
t350f4link				5,276,577			37.5 × 37.5 × 37.5 nm3		667 ns 350 K
pullf4link	1			5,276,577			37.5 × 37.5 × 37.5 nm3		1,956 ns 350 K
snfreet350	2			3,960,735			32.5 × 37.5 × 32.5 nm3		1,000 ns 350 K
nscpull4l	1			5,319,378			37.7 × 37.7 × 37.7 nm3		900 ns 350 K

^*All systems included four trans-SNARE complexes (except snfreet350 and nscpull4l), a 24 nm vesicle, and the indicated flat bilayer.