FIG. 1.

The resorcinol-positive pool from the Iatrobead column (up to 5 μmol of sialic acid) was injected onto a semipreparative silica HPLC column as described in Materials and Methods. The response from the evaporative light-scattering detector is shown (versus elution time). Aliquots of fractions were spotted onto TLC plates and sprayed with resorcinol to locate sialic acid-containing fractions, which are designated by the solid black bar on the figure. Resorcinol-positive fractions combined to form pools 1, 2, and 3 are indicated by brackets. (Inset) Between 10 and 20 nmol of sialic acid equivalents of each ganglioside pool or approximately 3 nmol of standard GM₃ or GM₂ was applied to a channeled silica gel plate, developed as described in Materials and Methods, and stained with resorcinol to visualize ganglioside bands. Lanes A and E, GM₃ and GM₂; lanes B, C, and D, HPLC pools 1, 2, and 3, respectively.