TABLE 3.
Reconstitution of rotavirus infectivity by NeuGcGM3a
| MA-104 cell treatment | Virus infectivity, FFU/well (mean ± SD)b
|
|
|---|---|---|
| Expt A | Expt B | |
| None | 615 ± 41 (100) | 934 ± 308 (100) |
| Nanase | 33 ± 10 (5) | 266 ± 127 (28) |
| Nanase + NeuGcGM3 | 90 ± 20 (15) | 438 ± 63 (47) |
a
MA-104 cells in quadruplicate wells of a 24-well plate were incubated with or without neuraminidase, washed, and further incubated with or without NeuGcGM3 as described in Materials and Methods. Following additional washing, the monolayers were incubated with 100 μl of purified porcine rotavirus TLP containing 1.7 (experiment A) or (experiment B) 17 ng of protein for 15 min. The plates were incubated and processed for measurement of FFU as described in the legend to Fig. 7 and in Materials and Methods.
b
Values in parentheses are percentages of control infectivity determined in the absence of neuraminidase treatment and added ganglioside.