TABLE 1.

Expression of vaccine plasmids in transfected Cos cells^a

Plasmid	Total amt of Env (ng)	% Env in supernatant	Amt of hGH in supernatant (ng)
pJW4303	0	NAc	316
pCMV	0	NA	502
pHXB2gp120	12	92	242
pHXB2gp140	15	49	302
pHXB2env	35	10	410
pHXB2dpolb	230	27	NDd

^aTissue culture media and cellular lysates were collected at 24 h and subjected to HIV-1 antigen capture ELISA. An hGH-expressing plasmid (pWR61602) was included as an internal control. This experiment is representative of repeated assays. 

^bExpression levels (in nanograms) of pHXB2dpol are high because the ELISA used detects total HIV-1 antigens rather than Env alone. 

^cNA, not applicable. 

^dND, not done.