TABLE 2.
Peak ELISA and IIIb-neutralizing titersa
Group | Rabbit | Anti-Env ELISA titers (μg of IgG/ml of serum)
|
IIIb-neutralizing titers
|
||||
---|---|---|---|---|---|---|---|
DNA | rgp160 × 1 | rgp160 × 2 | DNA | rgp160 × 1 | rgp160 × 2 | ||
Env | R104 | 0.3 ± 0.1 | 65 ± 12 | 1,150 ± 202 | <20 | NDb | 1,198 |
R105 | 0.4 ± 0.1 | 105 ± 14 | 1,693 ± 342 | <20 | 287 | 2,425 | |
R106 | 0.5 ± 0.1 | 70 ± 28 | 879 ± 263 | <20 | 199 | 1,963 | |
0.4 | 78 | 1,196 | 243 | 1,786 | |||
Env++ | R107 | 1 ± 0.2 | 101 ± 30 | 1,017 ± 331 | <20 | 411 | 546 |
R108 | 2 ± 0.8 | 81 ± 14 | 678 ± 219 | <20 | 500 | 2,560 | |
R109 | 4 ± 0.2 | 102 ± 34 | 762 ± 206 | 48 | 95 | 830 | |
0.9 | 94 | 807 | 269 | 1,050 | |||
dpol | R110 | 2 ± 0.6 | 187 ± 29 | 864 ± 212 | 105 | 291 | 18,369 |
R111 | 0.6 ± 0.1 | 50 ± 6 | 420 ± 100 | <20 | 47 | 2,829 | |
R112 | 0.8 ± 0.1 | 133 ± 33 | 568 ± 137 | <20 | 1,487 | 5,318 | |
0.8 | 108 | 590 | 272 | 6,516 | |||
dpol++ | R113 | 0.4 ± 0.1 | 131 | 674 ± 356 | <20 | 953 | 1,488 |
R114 | 3 ± 0.6 | 87 ± 12 | 393 ± 102 | 108 | 765 | 861 | |
R115 | 2 ± 0.1 | 107 ± 19 | 714 ± 296 | <20 | 228 | 886 | |
1 | 107 | 574 | 550 | 1,043 | |||
rgp160 only | R126 | 8 ± 2 | 176 ± 59 | 61 | 869 | ||
R127 | 28 ± 9 | 221 ± 58 | 102 | 1,174 | |||
R128 | 18 ± 4 | 229 ± 85 | 131 | 741 | |||
16 | 207 | 93 | 911 |
Peak DNA-elicited titers were determined 2 weeks after the third DNA priming (DNA), while boosted titers were determined 4 weeks after each protein boost (rgp160 × 1 and rgp160 × 2, first and second protein boosts with rgp160, respectively). All ELISAs were performed three times, and titers are expressed as arithmetic means ± standard deviations. IIIb-neutralizing titers (50% neutralization) of the same serum samples were determined on MT-2 cells by using a cell viability assay. Values in boldface type are GMTs. ELISA and neutralizing titers were determined either 2 or 4 weeks after the final DNA immunization or each protein immunization.
ND, this value was not determined.