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. 1998 Nov;72(11):9092–9100. doi: 10.1128/jvi.72.11.9092-9100.1998

TABLE 2.

Peak ELISA and IIIb-neutralizing titersa

Group Rabbit Anti-Env ELISA titers (μg of IgG/ml of serum)
IIIb-neutralizing titers
DNA rgp160 × 1 rgp160 × 2 DNA rgp160 × 1 rgp160 × 2
Env R104 0.3 ± 0.1 65 ± 12 1,150 ± 202 <20 NDb 1,198
R105 0.4 ± 0.1 105 ± 14 1,693 ± 342 <20 287 2,425
R106 0.5 ± 0.1 70 ± 28 879 ± 263 <20 199 1,963
0.4 78 1,196 243 1,786
Env++ R107 1 ± 0.2 101 ± 30 1,017 ± 331 <20 411 546
R108 2 ± 0.8 81 ± 14 678 ± 219 <20 500 2,560
R109 4 ± 0.2 102 ± 34 762 ± 206 48 95 830
0.9 94 807 269 1,050
dpol R110 2 ± 0.6 187 ± 29 864 ± 212 105 291 18,369
R111 0.6 ± 0.1 50 ± 6 420 ± 100 <20 47 2,829
R112 0.8 ± 0.1 133 ± 33 568 ± 137 <20 1,487 5,318
0.8 108 590 272 6,516
dpol++ R113 0.4 ± 0.1 131 674 ± 356 <20 953 1,488
R114 3 ± 0.6 87 ± 12 393 ± 102 108 765 861
R115 2 ± 0.1 107 ± 19 714 ± 296 <20 228 886
1 107 574 550 1,043
rgp160 only R126 8 ± 2 176 ± 59 61 869
R127 28 ± 9 221 ± 58 102 1,174
R128 18 ± 4 229 ± 85 131 741
16 207 93 911
a

Peak DNA-elicited titers were determined 2 weeks after the third DNA priming (DNA), while boosted titers were determined 4 weeks after each protein boost (rgp160 × 1 and rgp160 × 2, first and second protein boosts with rgp160, respectively). All ELISAs were performed three times, and titers are expressed as arithmetic means ± standard deviations. IIIb-neutralizing titers (50% neutralization) of the same serum samples were determined on MT-2 cells by using a cell viability assay. Values in boldface type are GMTs. ELISA and neutralizing titers were determined either 2 or 4 weeks after the final DNA immunization or each protein immunization. 

b

ND, this value was not determined.