FIG. 3.
(A) Incorporation of envelope proteins into retroviral vectors produced at 37°C. The upper blot was probed with anti-SU and anti-CA antibodies, and the lower blot was probed with anti-TM antibody. PR represents the precursor protein. (B) Envelope proteins in the lysates of transfected 293T cells produced at 37°C and detected with anti-TM antibody. (C) Incorporation of envelope protein into retroviral vectors produced at 32°C. The upper blot was probed with anti-SU and anti-CA antibodies, and the lower blot was probed with anti-TM antibody. Lanes No Env contain vectors produced without an env expression vector.