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Immunoaffinity purification of A3 supertype peptides. Cell lysates obtained from each of the indicated cell lines were sequentially run over immunoaffinity columns in the order indicated. The antibody used in each of the sequential columns is indicated, and is followed by the MHC class I molecule that was retained by that antibody. Peptides obtained from the MHC class I molecules in bold type were analyzed for the presence of specific peptides using SRM mass spectrometry.
