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Expression of gp100 and NY-ESO-1 genes in A3 supertype–expressing cell lines
| Gene | Cell line | |||||
|---|---|---|---|---|---|---|
| VMM12 (HLA-A3) | VMM18 (HLA-A3) | VMM19 (HLA-A11) | VMM86 (HLA-A31) | VMM150 (HLA-A68) | DM14 (HLA-A68) | |
| GAPDH | +a | + | + | + | + | + |
| gp100 | + | + | + | + | − | + |
| NY-ESO-1 | + | + | − | + | − | − |
aPlus sign Indicates that a band of an appropriate molecular weight was observed on a agarose gel following RT-PCR. A negative sign indicates that no such band was observed
