Table 5.

Detection of selected A3 supertype–associated peptides among HLA-A3 peptide extracts obtained from two different melanoma cell lines

Source protein	Peptide sequence	Known restriction element	Copy level of peptide per cell
			VMM12 (HLA-A3)	VMM18 (HLA-A3)
TAG	RLSNRLLLR	HLA-A3	+ [+]b	++ [+]
Unknown	SQNFPGSQK	HLA-A3	++ [?]c	++ [?]
TRP-2	LLGPGRPYR	HLA-A31	++ [+]	NTd

^a+, One to ten copies per cell; ++, greater than ten copies per cell and equal to or less than 20 copies per cell

^b[+] Indicates that the cell line was positive by PCR for the expression of the corresponding tumor antigen gene

^c[?] Indicates that the tumor antigen is derived from an unknown source protein, and thus it is not possible to determine by PCR those cell lines expressing the corresponding tumor antigen gene

^dNT not tested