Fig. 7.

Mice injected with C5a expressing cells display reduced cellularity before they show overt external signs of decreased tumor size. Mice were injected as in Fig. 6. At the indicated days, mice were measured and sacrificed. a Mean leg diameter for each group. b After the mice were sacrificed, the tumors were carefully excised and weighed. The mean weight of each group is presented. c A single cell suspension was made by treating the tumors with collagenase. Cells were quantitated by microscopy, counting only cells that excluded trypan-blue. The mean number of cells per tumor for each group is presented. Symbols, * P<0.05; ** P<0.01. Hatched bars, control tumors; solid bars, C5a tumors