Table 1.
Sequence analysis of positive clones from immunoscreening following biopanning with high and low IgG concentration. Identity of cDNA inserts of immunoreactive phages. H.p. hypothetical protein. Accession numbers of databank entry in italics
| Sequence identity | Number of clones isolated | 5’ nucleotidea | Reading frameb |
|---|---|---|---|
| High IgG concentration | |||
| NY-ESO-1 XM_049359 | 23 | 307/340 | 0 |
| NY-ESO-1sv AJ275978 | 44 | 171 | 0 |
| Lage-1 AJ223041 | 2 | 31/88 | 0 |
| Xage-1 NM_133431 | 2 | 510/542 | −1 |
| GlyII NM_005326 | 1 | 1,009 | 0 |
| GAPDH NM_002046 | 1 | 366 | −1 |
| C1qβ P02746 | 1 | 415 | +1 |
| H.p. with nudix/MutT motif BC18644 | 2 | 397/454 | 0 |
| Low IgG concentration | |||
| NY-ESO-1 XM_049359 | 30 | 307 | 0 |
| NY-ESO-1sv AJ275978 | 31 | 171/180 | 0 |
| Peflin XM_034479 | 4 | 187 | −1 |
| H.p. with nudix/MutT motif BC18644 | 1 | 397 | 0 |
| Nuclear autoantigen sp100 NM_003113 c | 1 | ||
| NADUOx NM_004548 | 1 | 23 | 0 |
| Human EST BF110350 d | 1 | 27 | |
| H.p. HSCARG AF225419 | 1 | 274 | +1 |
| H.p. MGC12943 NM_032317 | 1 | 529 | −1 |
aFirst 5’ nucleotide in databank entry matching pJuFo clone
bReading frame of databank entry relative to pJuFo pelBpIII sequence
cClone with internal deletion, start of insert and reading frame not known
dNo predicted reading frame