Fig. 3.

Effect of EF on apoptosis/necrosis. SW620 tumour cells were either untreated, mock fused, fused or freeze thawed prior to staining with Annexin-V-FITC versus PI and analysed by FACS. a Dot plots of Annexin-V-FITC versus PI staining following each treatment. Figures indicate the percentage of cells present in the upper right (Ann+/PI+) and lower right (Ann+/PI−) quadrants. Data are from a representative experiment of five performed. b Bar graph of the percentages of Ann+/PI− and Ann+/PI+ cells present following each treatment. Data are from five separate experiments and are shown as mean percent±SEM. ** indicates P<0.001 following comparison with untreated cells