Fig. 1.

Purification of gp96-peptide complexes from human HCC cell line SMMC-7721. a 12% reducing SDS-PAGE analysis of purified gp96-peptide complexes with silver staining. Gp96-peptide complexes were isolated from SMMC-7721 cells as described in Materials and methods; b immunoblotting analysis of purified gp96-peptide complexes. Gp96-peptide complexes were identified by anti- human gp96 antibody. Lane 1 represents the protein molecular marker and lane 2 represents human gp96-peptide complexes; c analysis of Con A contamination level in human gp96-peptide complexes by immunoblotting. Lane 1 represents the protein molecular marker, lane 2 represents positive control of Con A protein (10 ng) and lane 3 represents human gp96-peptide complexes (2 μg)