Fig. 4A–C.

Enhanced cytotoxicity of T-LAK cells or peripheral blood mononuclear cells (PBMCs) by Ex3 diabody in 6-h ⁵¹Cr release assay. A The TFK-1 cells were co-cultured with T-LAK cells at an E:T ratio of 10 in combination with various concentrations of Ex3 diabody. * p<0.01, ** p<0.001, and *** p<0.0001 for each group vs the control (without Ex3 diabody). B The TFK-1 cells were co-cultured with T-LAK cells (squares) or PBMCs (triangles) at various E:T ratios in the presence (closed symbols) or absence (open symbols) of Ex3 diabody (1.0 μg/ml). ** p<0.01, *** p<0.001, and **** p<0.0001 for the percentage of specific lysis by T-LAK cells or PBMCs in the presence of 1 μg/ml of Ex3 diabody vs in the absence of Ex3 diabody for each group. C Cytotoxicity to three bile duct carcinoma cell lines. TFK-1 (squares), OCUCh-LM1 (triangles), HuCC-T1 (circles) cells were co-cultured with T-LAK cells at various E:T ratios in the presence (closed symbols) or absence (open symbols) of Ex3 diabody (1.0 μg/ml). **, ##, †† indicate p<0.01, ***, ###, ††† indicate p<0.001, and ****, ####, †††† indicate p<0.0001 for the percentage of specific lysis of TFK-1, OCUCh-LM1, and HuCC-T1, respectively, in the presence of 1 μg/ml of Ex3 diabody vs in the absence of Ex3 diabody for each group. Data are representative of at least three independent experiments, using different donors, with similar results. Statistical analysis was carried out using the Student’s t test