Fig. 2a-h.

Indirect immunohistochemical staining of BLCA-38 expression in prostate cancer cell lines, xenografts in nude mice and human prostate tumour biopsies. Strong membrane and cytoplasmic staining were seen in a cytospins of the positive cell line DU-145, by green immunofluorescence; b paraffin sections of the DU-145 xenograft, by immunoperoxidase/DAB; c PC-3 xenografts grown s.c. detected by immunoalkaline phosphatase/fast red; d PC-3 lymph node metastases detected by immunoalkaline phosphatase/fast red. Inserts show negative controls, a stained with irrelevant isotype–matched MAbs or b,c,d procedure controls where no primary antibody was used. e Human prostate cancers of Gleason score 6 (frozen section), insert shows normal prostate (frozen section). f PIN in human prostate cancer specimen (formalin-fixed, paraffin-embedded). g Human prostate cancers of Gleason score 9 (formalin-fixed, paraffin-embedded). h Positive staining in intraductal carcinoma of the prostate, but with no staining of the basal cells, that stain positively for cytokeratin 34βE₁₂ (insert, formalin-fixed, paraffin-embedded). Original magnification of images: a,b,e–h (also inserts) at ×40; c,d (also inserts) at ×20