Fig. 2A, B.

Effect of sodium ascorbate on apoptosis as a prooxidant. A Effect of sodium ascorbate on ROS levels of B16F10 melanoma cells. Cells (2×10⁴ cells/well) were placed in wells of a 96-well microtiter plate followed by an addition of 50-μM 2', 7'-dichlorofluorescein diacetate at 37°C in a 5% CO₂ humidified incubator, and then analyzed with a Cytofluor 2350 plate reader with excitation at 485 nm and emission at 530 nm. Results are representative of more than five experiments, each performed in triplicate. The data are shown as the mean ± SD. B Effect of the antioxidant, N-acetyl-l-cysteine on sodium ascorbate–induced apoptosis. B16F10 melanoma cells were incubated with medium (control), sodium ascorbate (10 mM), or sodium ascorbate/N-acetyl-l-cysteine (10 mM) for 4 h. After incubation, cells were collected and stained with Annexin V-FITC to detect apoptosis of melanoma cells. Results are representative of more than five experiments