Fig. 2.

Schematic representation of a scFv (anti-HER-2/neu)-ζ or -γ construct. The Moloney murine leukemia virus 5′ long terminal repeat (LTR) controls the expression of the CAR scFv (HB8696) ζ or γ, which consists of an N-terminal heavy chain leader peptide (L), the HER-2/neu–specific single-chain antibody scFv (hb8696), an myc-tag (shown in Fig. 1), the hinge region of CD8 α chain, and the CD3 ζ or FcɛRI γ chain. The neomycin-resistance gene for G418 selection of transduced cells is driven by the SV40 early promoter