Fig. 1.

Western blot analysis of vaccine-induced IgG antibody responses at different time points. Five mice were immunized to a polyvalent melanoma vaccine (lanes 2–5) or vaccine admixed with alum (lanes 6–9) weekly ×4. Pooled sera were tested for antibodies against antigens in the vaccine at baseline (lanes 2 and 6) and 2 (lanes 3 and 7), 4 (lanes 4 and 8), and 6 weeks (lanes 5 and 9) after the first immunization. Lane 1 represents a no-serum negative control.