Fig. 1a, b.

Surface expression of CD80, CD86 on 3C cells. a Rhesus monkey 3C, 2V, 2S, and 3081 EBV transformed B cells were stained with anti-CD80 (clone 5B5), anti-CD86 (clone 1G10), or control IgG, followed by rabbit antihuman FITC-conjugated IgG. Results were analyzed by flow cytometry as indicated in the figure, and shown are the results for cell line 3C. b Cytotoxicity of αCD86-IT and αCD80-IT to rhesus monkey B cell lines. Rhesus monkey 3C cells were incubated with different concentrations αCD86-IT, αCD80-IT, or both, for 72 h and additional 16 h with ³H-leucine. Inhibition of protein synthesis is expressed as percentage of ³H-leucine incorporation by untreated cells. Concentrations refer to the amount of gelonin in the IT used. Gelonin alone did not induce significant inhibition of protein synthesis (not shown). Data represent mean of three independent experiments. Standard deviations were less than 10%