Table 1.

Mitogens and concentrations used throughout the experiments

Mitogen	Concentration
PHA (PHA-L, Sigma Aldrich, St Louis, MO, USA)	5 µg/ml
αCD3 (Orthoclon OKT3, Ortho Biotech Products, Raritan, NJ, USA)	12.5 ng/ml (soluble)
αCD3 (Orthoclon OKT3, Ortho Biotech Products, Raritan, NJ, USA)	1 µg/ml (plastic-bound)
αCD2 (T11 1/1, clone 6G4, and 2/1, clone 4B2) (CLB, Amsterdam, The Netherlands)	2.5 µg/ml
αCD28 (KOLT-2) (CLB, Amsterdam, The Netherlands)	50 ng/ml
PMA (Sigma Aldrich)	10 ng/ml when used alone, 1.2 ng/ml when combined with ionomycin
Ionomycin (Calbiochem, La Jolla, CA, USA)	1 µM
IL-2 (rhIL-2, TECIN, Hoffman-La Roche, Newton, NJ, USA)	50 U/ml