Fig. 2.

Identify peptides-specific CTLs by tetramer analysis PBMCs from normal HLA-A*0201 donor were stimulated with peptides (QLVQSGAEV)-loaded autologous PBMC as the APCs every week, the frequency of CD8 and QLVQSGAEV /HLA-A*0201 tetramer double positive cells were assessed by FACS. a. CTLs stimulated by IgHV1 peptide were stained with a HCV (FLLSLGIHL/ HLA-A*0201) tetramer as a negative control; the double positive frequency was 0.02%. b CTLs stimulated by FLU peptide were stained with a QLVQSGAEV/HLA-A*0201tetramer as a negative control; the double positive frequency was 0.04%. c Before IgHV1 peptide stimulation, the frequency of CD8 and QLVQSGAEV /HLA-A*0201 tetramer double positive cells was 0.38%. d After the second stimulation, the frequency was 1.55%. e After the third stimulation, the frequency was 13.93%. f After the fourth stimulation, the frequency was 49.38%