Fig. 3.

TCRβ receptor expression of CTL clone TCR BV gene usage was determined by RT-PCR, a1 Before the peptide stimulation, each TCR repertoire’s subfamily was expressed broadly. The PCR products were separated on 6% polyacrylamide gels. a2 For fingerprinting, the PCR products were separated on 5% denatured polyacrylamide gels containing 7 mol/l urea, and visualized by silver staining. Each subfamily’ amplification resulted in a number of bands that showed a Gaussian distribution. b After four stimulations, the TCR repertoires were skewed in several families. Ca (600 bp long-band) was used as an internal control