Fig. 5.

CTL induced IFN-γ secretion IFN-γ secretion was assayed after the IgHV1(QLVQSGAEV)peptide special-CTL lines co-culture with several tumor cell lines: NAMALWA (HLA-A*0201-positive, IgHV1-positive), THP-1 (HLA-A*0201ositive, IgHV1-negative), K562 (HLA-A*0201-nagative, IgHV1-negative), T2 (HLA-A*0201-positive). Overnight co-culture supernatants were assayed for IFN-g secretion with the ELISA method. Data are expressed as the mean ± SEM of three independent experiments. The peptide unloaded-T2 cells as the target cells were used as the control group to determine statistical significance. *Significant secretion. (P<0.05). V1: IgHV1 (QLVQSGAEV) peptide