Fig. 2a–c.

Western blot analysis of PSA-HSP chimeric protein expression. MC57 cells were transfected with the different PSA-HSP–expressing constructs and cultured for 10 h in the presence (“+”) or absence (“−”) of GolgiPlug reagent. PSA or PSA-HSP fusion proteins were detected with antibodies against human PSA, in total cell lysates (a) and culture supernatant (b–c). The loaded samples are numbered in accordance with Fig. 1