Fig. 3a, b.

Detection of PSA-specific lytic activity in mice immunized with PSA-HSP plasmids. a Splenocytes were restimulated for 5 days with EL4-PSA cells and then tested against EL4 (open circles) or EL4-PSA targets (solid circles) in a 5-h cytotoxicity assay. A representative experiment is shown for one mouse from each immunization group. b Comparison of PSA-specific lytic activity in mice immunized with the different PSA-HSP plasmids and then assayed against EL4 or EL4-PSA targets in a cytotoxicity assay. The mean value from four mice analyzed individually and standard deviation are shown. The immunization groups are numbered in accordance with Fig. 1