Fig. 4a, b.

Quantification of PSA-specific CD8⁺ T cells in mice immunized with PSA-HSP plasmids. After 5 days of in vitro restimulation with EL4-PSA cells, splenocytes were incubated with B16 or B16-PSA tumor cells, stained with antibodies against CD8 and IFN-γ, and analyzed by flow cytometry. a A representative experiment is shown for one mouse from each immunization group. b Quantitative comparison of PSA-specific CD8⁺ T cells in mice immunized with the different PSA-HSP plasmids. The mean value from four mice analyzed individually and standard deviation are shown. Immunization groups are numbered in accordance with Fig. 1