Fig. 5A–D.

Cytotoxicity assay with in vitro expanded peptide-specific CTL. CD8⁺ cells were stimulated in vitro with matured autologous DC pulsed with one of the PSA-derived peptides. Cytotoxicity was measured by ⁵¹Cr release from labeled different target cells. Target cells were T2 cells alone, T2 cells pulsed with one of the four PSA peptides, T2 cells pulsed with Flu peptide, HLA-A2 positive and PSA positive prostate cancer cell line LN CaP, and HLA-A2 positive and PSA negative melanoma cell line CS-M. A CTL activity with responding CD8⁺ cells stimulated with DC pulsed with PSA-1. B CTL activity with responding CD8⁺ cells stimulated with DC pulsed with PSA-2. C CTL activity with responding CD8⁺ cells stimulated with DC pulsed with PSA-3. D CTL activity with responding CD8⁺ cells stimulated with DC pulsed with PSA-4. Solid square % specific lysis of the target T2 cells alone. Solid circle % specific lysis of the target T2 cells pulsed with peptide PSA-1. Solid triangle % specific lysis of the target T2 cells pulsed with peptide PSA-2. Solid diamond % specific of the target T2 cells pulsed with peptide PSA-3. Open square % specific lysis of the target T2cells pulsed with peptide PSA-4. Open circle % specific lysis of the target T2 cells pulsed with Flu peptide. Open triangle % specific lysis of the target LN CaP tumor cells. X % specific lysis of HLA A2 positive melanoma tumor target CS-M cells