Table 2.

Mean fluorescence index of DC markers after immunophenotypic analysis of uncultured AML blasts and cultured AML-APCs in three different media

MFI cytokine culture
	CD40				CD54				CD80
	Day 0	FCS	SP	CG	Day 0	FCS	SP	CG	Day 0	FCS	SP	CG
Mean	4.5	15.7	13.1	11.7	3.1	7.7	7.7	5.2	0.8	5.2	4.4	3.7
SEM	1.1	2.8	1.9	2.8	0.5	1.8	1.6	0.8	0.1	1.1	0.8	1.4
Median	3.2	10.8	11.7	9.1	2.1	5.7	6.1	3.8	0.8	3.2	2.8	1.6
	CD83				CD86				HLA-DR
	Day 0	FCS	SP	CG	Day 0	FCS	SP	CG	Day 0	FCS	SP	CG
Mean	0.9	1.1	1.7a	1.0	2.7	3.6	3.4	3.6	15.8	9.0	14.3	15.9
SEM	0.1	0.1	0.5	0.1	0.6	0.6	0.6	0.9	4.5	2.1	2.9	6.5
Median	0.9	0.9	1.2	0.9	1.4	2.8	2.6	2.0	12.8	5.6	11.3	9.6
MFI CI-culture
	CD40				CD54				CD80
	Day 0	FCS	SP	CG	Day 0	FCS	SP	CG	Day 0	FCS	SP	CG
Mean	4.5	47.2	47.3	24.2	3.1	37.3	36.7	13.0	0.8	23.8	25.2	7.9
SEM	1.1	12.5	11.0	6.4	0.5	11.1	10.4	4.0	0.1	8.5	8.1	2.8
Median	3.2	25.1	28.5	16.0	2.1	17.7	17.9	8.6	0.8	8.3	8.4	4.1
	CD83				CD86				HLA-DR
	Day 0	FCS	SP	CG	Day 0	FCS	SP	CG	Day 0	FCS	SP	CG
Mean	0.9	7.8	8.4	2.6	2.7	27.4	28.9	6.8	15.8	40.9	38.4	29.0
SEM	0.1	3.2	2.8	0.8	0.6	8.1	11.1	1.9	4.5	13.3	11.6	15.7
Median	0.9	3.3	3.0	1.9	1.4	16.8	10.9	6.5	12.8	24.3	19.3	13.4

^a P <0.05 as compared to CG

CI calcium ionophore.

FCS FCS-containing medium (cytokine culture n=17, CI culture n=14).

SP StemSpan serum-free medium (cytokine culture n=19, CI culture n=17).

CG CellGro serum-free medium (cytokine culture n=13, CI culture n=8).