Fig. 5.

Lm-LLO-E7 enhances T-lymphocyte trafficking to both IFN-γ–sensitive and IFN-γ–insensitive tumors. Naïve C57BL/6 mice were injected subcutaneously with Matrigel containing 1×10⁵ TC1 or 1×10⁵ TC1.mugR tumor cells. On day 7, mice were injected i.p. with either PBS or Lm-LLO-E7. Seven days after treatment, Matrigel plugs were harvested and weighed. Single-cell suspensions were prepared, and the number of live cells per gram of tissue determined by trypan blue exclusion. The presence of CD8⁺ T cells (A, B) and CD4⁺ T cells (C, D) was analyzed by flow cytometry. The number of CD8⁺ and CD4⁺ T cells per gram of tissue was calculated by multiplying the cell population percentage for CD8⁺ or CD4⁺ cells by the determined number of live cells per gram of tissue. The presence of CD8⁺ T cells (a) and CD4⁺ (B) T cells were analyzed by flow cytometry. Data are mean percentage of live cells ± SEM (A, C) and number of live cells per gram of tissue (C, D); asterisks paired measurements that are statistically different as determined by Student’s t test.