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BALB-neuT mice were vaccinated with different plasmids, coding for various portions of the rat p185neu protein. All cDNA were cloned into a pcDNA3 vector. The most effective plasmid was the one coding for the ECD-TM domain of rat p185neu. The plasmids coding for the full length and ECD plus IL-1β were less effective, while the ECD alone was the least effective
