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Longer protection by DNA vaccination and mLAG-3Ig. BALB-neuT mice were immunized with 100-μg ECD-TM plasmid alone or in association with 20 μg of mLAG-3Ig. At 52 weeks of age, 20% of mice vaccinated with ECD-TM and about 80% of those with ECD-TM + mLAG-3Ig are tumor free