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. 2005 May 13;54(11):1059–1071. doi: 10.1007/s00262-005-0671-8

Fig. 1.

Fig. 1

Construction of Bs-Abs and CriBs-Abs. a Overview of the gene constructs for expression of the activating and co-stimulating Bs-Ab: the E6 Fab light chain gene (E6L) (i) and the E6 Fab heavy chain gene (E6H) extended with the gene encoding an anti-murine CD3ε scFv (ii) or an anti-murine CD28 scFv (iii). b Co-expression of Fab light chain genes and Fab heavy chain scFv fusion genes leads to the production of an activating and a co-stimulating Bs-Ab. c Overview of the gene constructs for expression of the activating and co-stimulating CriBs-Abs: the E6 Fab light chain gene (E6L) extended with the DNA encoding PSTAIRE peptide (P) (iv) or the anti-P scFv (v) and the E6 Fab heavy chain gene (E6H) extended with the gene encoding an anti-murine CD3ε scFv (ii) or an anti-murine CD28 scFv (iii). d Co-expression of Fab light chain scFv fusion genes and Fab heavy chain scFv fusion genes, leads to the production of an activating and a co-stimulating CriBs-Ab. e Molecular model (ribbon representation) of the CriBs-Ab complex