Fig. 2.
Purification of the Bs-Abs and the CriBs-Abs. a Purification of the activating Bs-Ab: chromatogram with integrated molecular weight calibration curve of the polishing step with size exclusion chromatography. A slot blot of the different fractions was developed with a goat anti-mouse κ light chain antibody, followed by an alkaline phosphatase conjugated anti-goat antibody. Purification of the other Bs-Abs and CriBs-Abs gave similar chromatograms (data not shown). M monomers; D dimers; V0 void. b SDS-page (CBB stained) of the purified Bs-Abs and CriBs-Abs. Lanes: 1 activating Bs-Ab; 2 costimulating Bs-Ab; 3 activating CriBs-Ab; 4 costimulating CriBs-Ab