Fig. 5.

Induction of T-cell activation in the absence of tumor cells. T cells were co-cultured with (1) 80 nM of soluble activating and co-stimulating CriBs-Abs, (2) 80 nM of soluble activating and co-stimulating Bs-Abs, (3) 80 nM of soluble activating and co-stimulating Bs-Abs externally cross-linked via an anti-mouse kappa light chain polyclonal Ab, (4) 80 nM soluble anti-CD3 and anti-CD28 mAbs, or (5) 80 nM of soluble activating and co-stimulating CriBs-Abs with 400 nM of blocking peptide. The data are representative of three independent experiments